Literature summary for 1.3.99.31 extracted from
Seo, Y.; Choi, S.; Nam, S.; Kim, G.
Molecular cloning and characterization of the gene encoding phytoene desaturase from Kocuria gwangalliensis (2017), Microbiol. Biotechnol. Lett., 45, 226-235 .
No PubMed abstract available
Cloned(Commentary)
Cloned (Comment) |
Organism |
gene crtI, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic tree, recombinant enzyme expression from plasmid pCcrt1 in Escherichia coli. Lycopene biosynthesis is confirmed when the plasmid pCcrtI is cotransformed into Escherichia coli containing the plasmid pRScrtEB carrying the crtE and crtB genes required for lycopene biosynthesis |
Kocuria gwangalliensis |
Protein Variants
Protein Variants |
Comment |
Organism |
additional information |
lycopene biosynthesis is confirmed when the plasmid pCcrtI is cotransformed into Escherichia coli containing the plasmid pRScrtEB carrying the crtE and crtB genes required for lycopene biosynthesis |
Kocuria gwangalliensis |
Organism
Organism |
UniProt |
Comment |
Textmining |
Kocuria gwangalliensis |
H2DDY5 |
- |
- |
Subunits
Subunits |
Comment |
Organism |
? |
x * 57000, recombinant CrtI, SDS-PAGE |
Kocuria gwangalliensis |
Synonyms
Synonyms |
Comment |
Organism |
CrtI |
- |
Kocuria gwangalliensis |
phytoene desaturase |
- |
Kocuria gwangalliensis |
General Information
General Information |
Comment |
Organism |
evolution |
the nucleotide sequence of the crtI gene compared with that of other species, including Kocuria rhizophila and Myxococcus xanthus, proves well conserved during evolution |
Kocuria gwangalliensis |
metabolism |
the enzyme is involved in the lycopene biosynthetic pathway, overview |
Kocuria gwangalliensis |
physiological function |
phytoene desaturase (CrtI), encoded by the gene crtI, catalyzes lycopene formation from phytoene and is an essential enzyme in the early steps of carotenoid biosynthesis. CrtI is one of the key enzymes regulating carotenoid biosynthesis and has been implicated as a rate-limiting enzyme of the pathway in various carotenoid synthesizing organisms |
Kocuria gwangalliensis |